![]() Establishment of cell lines derived from different HCC patients is therefore useful for comprehensive study of pathogenesis of the disease. The heterogeneous nature of HCC has impeded patient prognosis and treatment. ![]() It is a highly heterogeneous disease in terms of its multiple molecular profiles and varied clinical outcomes. Hepatocellular carcinoma (HCC) is one of the most common and aggressive human malignancies worldwide. Subcutaneous injection of HCC21 cells into immunodeficient mice showed that the cells were able to form tumors at the primary injection sites and metastatic tumors in the peritoneal cavity. Sequence analysis revealed p53 gene mutation. The cell line was authenticated by short tandem repeat analysis. The cells exhibited typical epithelial morphology and expressed albumin, AFP and HBV antigens. The line, designated HCC21, derived from a Hong Kong Chinese female patient with HCC at Stage II. Cells from HCC patient 21 grew and expanded rapidly in vitro and was selected to be further characterized. GEP expression levels significantly correlated with the viability of isolated hepatocytes and success rate of subsequent primary culture establishment. ![]() 7 out of 16 cases (44%) could give rise to cells that were able to attach and grow in culture. Cells isolated from 16 out of 30 HCC cases (53%) had viability more than 70% and were subject to subsequent in vitro culture. ![]()
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